How the test is performed: Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and cause the vein to swell with blood.
A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children:
The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. A bandage may be applied to the puncture site if there is any bleeding.
Serology refers to the study of serum for its antibody content. An antibody defends the body against bacteria, viruses, fungus, or other foreign bodies (antigens). Certain microbial cells stimulate the body to produce these antibodies during an active infection. Antibody production increases during the course of an infection.
In the initial stage of an illness, few antibodies may be detected. For this reason, serology tests are often repeated several weeks after the initial sample. In the laboratory, the antibodies react with antigens in specific ways that can be used to confirm the identity of a specific microorganism.
There are several serology techniques that can be used depending on the suspected antibodies. Serology techniques include agglutination, precipitation, complement-fixation, and fluorescent antibodies.
How the test will feel: When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.
